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Measuring the Kinetics of Biomolecular Recognition with Magnetic Colloids

Cohen-Tannoudji, L. and Bertrand, E. and Baudry, J. and Robic, C. and Goubault, C. and Pellissier, M. and Johner, A. and Thalmann, F. and Lee, N. K. and Marques, C. M. and Bibette, J.

Thursday 25 June 2009, by mcube

We introduce a general methodology based on magnetic colloids to study the recognition kinetics of tethered biomolecules. Access to the full kinetics of the reaction is provided by an explicit measure of the time evolution of the reactant densities. Binding between a single ligand and its complementary receptor is here limited by the colloidal rotational diffusion. It occurs within a binding distance that can be extracted by a reaction-diffusion theory that properly accounts for the rotational Brownian dynamics. Our reaction geometry allows us to probe a large diversity of bioadhesive molecules and tethers, thus providing a quantitative guidance for designing more efficient reactive biomimetic surfaces, as required for diagnostic, therapeutic, and tissue engineering techniques.